Dietzgen, R.G. and Callaghan, B. and Wetzel, T. and Dale, J.L. (2006) Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus. Virus Research, 118 (1-2). pp. 16-22.
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Article Link(s): http://dx.doi.org/10.1016/j.virusres.2005.10.024
We completed the genome sequence of Lettuce necrotic yellows virus (LNYV) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, M (matrix protein), G (glycoprotein) and L (polymerase) genes. The genome consists of 12,807 nucleotides and encodes six genes in the order 3′ leader-N-4a(P)-4b-M-G-L-5′ trailer. Sequences were derived from clones of a cDNA library from LNYV genomic RNA and from fragments amplified using reverse transcription-polymerase chain reaction. The 4a protein has a low isoelectric point characteristic for rhabdovirus phosphoproteins. The 4b protein has significant sequence similarities with the movement proteins of capillo- and trichoviruses and may be involved in cell-to-cell movement. The putative G protein sequence contains a predicted 25 amino acids signal peptide and endopeptidase cleavage site, three predicted glycosylation sites and a putative transmembrane domain. The deduced L protein sequence shows similarities with the L proteins of other plant rhabdoviruses and contains polymerase module motifs characteristic for RNA-dependent RNA polymerases of negative-strand RNA viruses. Phylogenetic analysis of this motif among rhabdoviruses placed LNYV in a group with other sequenced cytorhabdoviruses, most closely related to Strawberry crinkle virus.
|Additional Information:||© Elsevier Science.|
|Keywords:||Plant rhabdovirus; LNYV genomic RNA; phylogeny; virology; vesicular; stomatitis virus; polyadenylated RNAs; stunt rhabdovirus; nucleocapsid gene; net virus; identification; proteins; plant; organization; variability.|
|Subjects:||Science > Microbiology > Virology|
Science > Biology > Genetics
Agriculture > Plant pests and diseases
Plant pests and diseases
|Deposited On:||30 Jan 2009 03:06|
|Last Modified:||10 Mar 2011 00:22|
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