Urech, R. and Brown, G. and Moore, C. and Green, P. (2005) Cuticular Hydrocarbons of Buffalo Fly, Haematobia exigua, and Chemotaxonomic Differentiation from Horn Fly, H.irritans. Journal of Chemical Ecology, 31 (10). pp. 2451-2461.
Article Link(s): http://dx.doi.org/10.1007/s10886-005-7112-1
Publisher URL: http://www.springerlink.com
We determined the quantity and chemical composition of cuticular hydrocarbons of different strains, sex and age of buffalo flies, Haematobia exigua. The quantity of cuticular hydrocarbons increased from less than 1 µg/fly for newly-emerged flies to over 11 µg/fly in 13 d-old flies. The hydrocarbon chain length varied from C21 to C29, with unbranched alkanes and monounsaturated alkenes the major components. Newly emerged flies produced almost exclusively C27 hydrocarbons. Increasing age was accompanied by the appearance of hydrocarbons with shorter carbon chains and an increase in the proportion of alkenes. 11 Tricosene and 7-tricosene were the most abundant hydrocarbons in mature buffalo flies. Cuticular hydrocarbons of buffalo flies are distinctly different from those of horn flies. The most noticeable differences were in the C23 alkenes, with the major isomers 11- and 7-tricosene in buffalo flies and (Z)-9- and (Z)-5-tricosene in horn flies, respectively. Cuticular hydrocarbon analysis provides a reliable method to differentiate buffalo and horn fly, which are difficult to separate morphologically. The differences in cuticular hydrocarbons also support their recognition as separate species, H. exigua and H. irritans, rather than as subspecies.
|Additional Information:||Author version © Queensland Department of Primary Industries and Fisheries. Reproduced in accordance with the copyright policy of the publisher. © Springer Science+Business Media B.V. Access to published version may be available via Publisher’s website.|
|Keywords:||Cuticular hydrocarbons; Haematobia; buffalo fly; horn fly; Diptera; chemotaxonomy; gas chromatography; mass spectrometry; alkenes.|
|Subjects:||Science > Physiology > Insect biochemistry|
|Deposited On:||14 Nov 2005|
|Last Modified:||07 Jun 2011 02:38|
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