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The use of PCR to aid in the rapid identification of Vibrio harveyi isolates

Oakey, H.J. and Levy, N. and Bourne, D.G. and Cullen, B. and Thomas, A. (2003) The use of PCR to aid in the rapid identification of Vibrio harveyi isolates. Journal of Applied Microbiology, 95 (6). pp. 1293-1303.

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Article Link(s): http://dx.doi.org/10.1046/j.1365-2672.2003.02128.x

Publisher URL: http://www.blackwell-synergy.com

Abstract

Aims: Vibrio harveyi is an important pathogen, causing potential devastation to marine aquaculture. This organism, however, is extremely difficult to identify because it is phenotypically diverse. Biochemical identification can involve many tests and take weeks to perform. The aim of this work is to develop a PCR that can reduce the number of biochemical tests, and the time taken, to get a definitive identification of this organism.

Methods and Results: The PCR was developed using 16S rDNA sequences from a number of V. harveyi strains, and other vibrios. The described test gave positive results for all strains of V. harveyi tested. However, some strains of V. alginolyticus also gave positive results and a small number of biochemical tests were required to differentiate between these two species. This indicated that preisolation of the bacteria was needed and therefore the test was not applicable to the testing of mixed populations directly.

Conclusion, Significance and Impact of the Study: The duration of identification of this species was significantly reduced from a number of weeks to a few days. Hence, diagnosis of affected animals will be faster and earlier treatment can be administered which may increase the survival rate from vibriosis.

Item Type:Article
Additional Information:© Blackwell Publishing Ltd. Journal subscribers may be able to access the published version of this article. Use hypertext link above to access publisher’s web site.
Keywords:16S rDNA; PCR; Vibrio alginolyticus; V. harveyi.
Subjects:Aquaculture and Fisheries > Aquaculture > Fish culture > Diseases and adverse factors
Deposited On:11 Jun 2004
Last Modified:03 Sep 2010 05:44

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